| Tara Strong | Twilight Sparkle (voice) |
| Andrea Libman | Pinkie Pie / Fluttershy (voice) |
| Ashleigh Ball | Rainbow Dash / Applejack (voice) |
| Tabitha St. Germain | Rarity / Princess Luna / Granny Smith / Mrs. Cake (voice) |
| Cathy Weseluck | Spike / Mayor Mare (voice) |
| Michelle Creber | Apple Bloom (voice) |
| Claire Margaret Corlett | Sweetie Belle (voice) |
| Madeleine Peters | Scootaloo (voice) |
| Nicole Oliver | Princess Celestia / Cheerilee (voice) |
| Peter New | Big McIntosh (voice) |
| Brenda Crichlow | Zecora (voice) |
| Chantal Strand | Diamond Tiara (voice) |
| Shannon Chan-Kent | Silver Spoon (voice) / Pinkie Pie (singing) |
| Brian Drummond | Mr. Cake (voice) |
| Rebecca Shoichet | Twilight Sparkle (singing) |
| Kazumi Evans | Rarity (singing) |
| William Shatner | Grand Pear (voice) |
| Bill Mondy | Burnt Oak (voice) |
| Peter New | Goldie Delicious (voice) |
| Felicia Day | Pear Butter (voice) |
| Bill Newton | Bright Mac (voice) |